Kumenya ingano ya aside oxalic mu mbuto n'imboga hakoreshejwe chromatography y'amazi ikora neza (HPLC)
1. Ihame ry'igerageza n'uburyo bwo gushushanya
Nk’aside organic isanzwe mu mbuto n’imboga, ingano ya aside oxalic igira ingaruka zitaziguye ku buryohe n’akamaro k’intungamubiri by’ibiryo. Iri gerageza rikoresha chromatography y’amazi ifite imikorere myiza (RP-HPLC). Mu gihe cy’imiterere y’aside mobile phase, aside oxalic itandukanyijwe n’ibintu biyibangamira hakoreshejwe inkingi ya chromatography ya C18. Imashini ipima imirasire ya ultraviolet kuri 210 nm ikoreshwa mu gusesengura ingano, hashingiwe ku miterere y’imirasire ya UV mu matsinda ya carboxyl mu molecule za aside oxalic.
2. Uburyo bwo gushushanya no gutegura icyitegererezo gisanzwe
Uburyo bwo gutandukanya ibintu hakoreshejwe gradient bukoreshwa mu gutegura ibisubizo bisanzwe: gupima neza 25.0 mg ya aside oxalic dihydrate hanyuma ugashyiramo 25 mL n'amazi meza cyane kugira ngo ubone 1 mg/mL. Ibi bishyirwamo ibisubizo bisanzwe kuri 50, 100, 200, 400, na 800 µg/mL, buri gipimo kikajyamo gatatu.
Mu gutegura icyitegererezo, hakoreshwa uburyo bwo gukuramo amazi hakoreshejwe mikoroonde: pima garama 5.00 z'imbuto/imboga, ongeramo mL 10 z'umuti wa aside hydrochloric wa 0.1 mol/L, shyira muri mikoroonde kuri dogere selisiyusi 60 mu gihe cy'iminota 10, yungurura unyuze muri filter ya membrane ya 0.45 µm, hanyuma ufate filtrate kugira ngo ikoreshwe.
3. Kunoza Imiterere ya Chromatographic
Icyiciro cyo kugenda ni sisitemu ya 0.01 mol/L ya potasiyumu dihydrogen phosphate buffer (pH 2.5)–acetonitrile (95:5), ifite umuvuduko w’amazi wa 0.8 mL/min n’ubushyuhe bw’inkingi bwa 35°C. Guhindura igipimo cya acetonitrile bigaragaza ko iyo igice cy’ibinyabuzima kirenze 10%, igihe cyo kubika aside oxalic kigabanuka kikagera ku minota itarenze 3, ariko habaho guhagarara cyane. Mu bihe bya nyuma, igihe cyo kubika aside oxalic ni iminota 4.2, bigatuma itandukana burundu na aside citric na aside malic yegeranye (resolution > 1.5).
4. Kwemeza uburyo
Igenzura ry’umurongo rigaragaza isano iri hagati y’ubuso bw’agasongero n’ubwinshi buri hagati ya 10–1000 µg/mL (R² = 0.9993). Umupaka wo gutahura (LOD), ugenwa n’uburyo bwo gupima urusaku hagati y’ibimenyetso, ni 0.5 µg/mL. Mu bizamini byo gusubiramo, RSD ku bipimo bitandatu by’icyitegererezo kimwe cya epinari ni 1.8%. Igerageza ryo kugarura ingano ku rwego rutatu (80%, 100%, na 120%) ritanga impuzandengo yo kugarura ingano ya 98.2%, 102.4%, na 97.8%, uko bikurikirana, byujuje ibisabwa mu isesengura ry’imibare.
5. Isesengura ry'Ingero Nyazo
Imbuto n'imboga bitandatu biboneka ku isoko byageragejwe: epinari (356 ± 12 mg/100 g), seleri (215 ± 9 mg/100 g), inyanya (18 ± 2 mg/100 g), pome (6 ± 1 mg/100 g), imineke (ntibyabonetse), na broccoli (89 ± 5 mg/100 g). Igereranya n'uburyo busanzwe bw'igihugu (GB 5009.277-2016) rigaragaza ko itandukaniro riri hagati y'uburyo bubiri riri munsi ya 5%, rikemeza ko ubu buryo ari ubw'ukuri. By'umwihariko, uburyo bwa HPLC bugaragaza ubushobozi bwo kumva neza kurusha uburyo busanzwe bwo gusuzuma ingero z'ibipimo bike.
6. Ingingo z'ingenzi
Genzura neza agaciro ka pH mu gihe cyo kuvura icyitegererezo. Niba pH y'ikinyabutabire irenze 3, imvura ya calcium oxalate izatuma habaho ibisubizo bike by'ibizamini. Igice cyo kunyuramo kigomba gutegurwa buri munsi kugira ngo hirindwe imvura y'umunyu no kuziba kw'inkingi. Nyuma ya buri gutera inshinge 20, oza inkingi n'amazi ya acetonitrile (30:70) mu gihe cy'iminota 30 kugira ngo hirindwe kwangirika kw'imikorere y'inkingi. Ku ngero zirimo ibara (urugero: ikayi y'umuhengeri), ni byiza kongeramo intambwe yo gukaraba ikoreshwa mu gukuramo intanga mbere yo gutera inshinge.
7. Amabwiriza yo Gukoresha no Kwagura
Ubu buryo bushobora kwaguka mu gupima aside oxalic mu bipimo bya binyabuzima nk'inkari n'amaraso. Mu guhindura imiterere y'urwego rw'umubiri (urugero, kongeramo tetrabutylammonium hydroxide), aside oxalic na metabolites zayo bishobora kumenyekana icyarimwe. Hamwe n'icyuma gipima imiterere y'ibice, hashobora gushyirwaho uburyo bwo kumenya ibimenyetso neza. Mu nganda zitunganya ibiribwa, ubu buryo bushobora gutanga inkunga y'ingenzi mu guhitamo ubwoko bw'aside oxalic nke no kunoza uburyo bwo guteka.
Zibo Anhao Chemical Co., Ltd. – aside oxalic
Zibo Anhao Chemical Co., Ltd. ni umucuruzi wizewe kandi wohereza mu mahanga aside oxalic nziza cyane (ifite urwego rw'inganda, 99.6% min) ikomoka i Shandong, mu Bushinwa. Aside oxalic yacu ni crystalline solid yera, izwi kandi nka aside ethanedioic, ifite formula ya molekile C₂H₂O₄. Ifite ubuziranenge bwinshi, umwanda muke, kandi ifite ubuziranenge buhamye mu byiciro bitandukanye, ihura n'ibikenewe mu myenda, uruhu, ibyuma, gutunganya ubutaka budasanzwe, gusukura imiti, n'ubwubatsi.
Dutanga aside oxalic anhydrous na dihydrate, iboneka mu mifuka ya 25kg, imifuka ya 50kg, na 1000kg. Dukoresheje uburyo buhamye bwo gusuzuma, raporo zuzuye z'ibizamini, na gahunda zo gutanga ibicuruzwa zihindagurika, twemeza ko ibicuruzwa byacu bitangwa neza kandi bigakomeza gukora neza ku baguzi ku isi. Ibicuruzwa byacu byoherejwe cyane muri Aziya y'Amajyepfo y'Uburasirazuba, i Burayi, Afurika, na Amerika, bihabwa ishimwe kubera ubwiza bwizewe n'ibiciro bishimishije.
Hitamo Zibo Anhao Chemical kugira ngo ubone aside oxalic yizewe kandi utange serivisi z'ubuhanga ku miti imwe imwe.
Hitamo Zibo Anhao Chemical kugira ngo ubone aside oxalic nziza cyane itanga imikorere ihamye n'agaciro ku bucuruzi bwawe. Twandikire kugira ngo ubone amabwiriza menshi cyangwa ibisobanuro bya tekiniki: info@anhaochemical.com
